Molecular viability testing of viable but non-culturable bacteria induced by antibiotic exposure
نویسندگان
چکیده
منابع مشابه
Viable but Non-culturable Bacteria: Clinical Practice and Future Perspective
In this editorial, the importance of VBNC in clinical practice and future researches interests has been pinpointed. Accurate detection of VBNC, especially for major mentioned pathogens, is the future research gap.
متن کاملViable but non-culturable bacteria: their impact on public health
More than 50 years ago, standard microbiological methods were developed to determine whether bacterial cells were dead or alive. This is an important question because the answer is the basis of decisions such as safety of food and drinking water, sterility of pharmaceuticals, recurrence of infectivity and spoilage of treated industrial products. The viability of a bacterial cell was traditional...
متن کاملCurrent Perspectives on Viable but Non-Culturable (VBNC) Pathogenic Bacteria
Under stress conditions, many species of bacteria enter into starvation mode of metabolism or a physiologically viable but non-culturable (VBNC) state. Several human pathogenic bacteria have been reported to enter into the VBNC state under these conditions. The pathogenic VBNC bacteria cannot be grown using conventional culture media, although they continue to retain their viability and express...
متن کاملDetection of Viable But Non-Culturable State of Escherichia coli O157:H7 Using Reverse Transcription PCR
Background and Aims: Many bacteria including Escherichia coli may enter into a viable but non-culturable (VBNC) state under unfavorable stresses, which are unable to be detected by culture-based methods. In this study, the use of Reverse Transcription PCR (RT-PCR) for detection of VBNC state of E. coli O157:H7 was investigated. Materials and Methods: Escherichia. coli O157:H7 was inoculated i...
متن کاملAntibiotic pressure can induce the viable but non-culturable state in Staphylococcus aureus growing in biofilms.
OBJECTIVES Staphylococcal biofilms are among the main causes of chronic implant-associated infections. We have recently suggested that their transformation into viable but non-culturable (VBNC) forms (i.e. forms capable of resuscitation) could be responsible for the recurrent symptoms. This work aims to establish whether Staphylococcus aureus biofilms can give rise to VBNC forms capable of bein...
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ژورنال
عنوان ژورنال: Microbial Biotechnology
سال: 2017
ISSN: 1751-7915
DOI: 10.1111/1751-7915.13039